Design : viable mtb were detected in mesenteric lymph nodes , spleen and lungs at day 15 , 30and 60 after intragastric administration of bcg to mice : the proliferation of t - lymphocyte with the stimulus of the purified protein derivative ( ppd ) was measured at day 60 and 90 after intragastric administration of bcg to mice ; the expression of interleukin - 2 ( il - 2 ) of t - lymphocyte in spleen was detected at day 60 and 90 after intragastric administration of bcg 方法：在小鼠胃内接种bcg的第15 ， 30 ， 60天，检测结核杆菌在小鼠体内脏器的定植；接种bcg的第60 ， 90天，检测小鼠脾淋巴细胞受到ppd刺激后转化试验小鼠脾淋巴细胞受到ppd刺激后il - 2表达量。
